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O Brother, Where Art Thou? is the soundtrack album of music from the 2000 American film of the same name, written, directed and produced by the Coen Brothers and starring George Clooney, John Turturro, Tim Blake Nelson, and John Goodman.

The film is set in Mississippi during the Great Depression. The soundtrack, produced by T-Bone Burnett, uses bluegrass, country, gospel, blues, and Southern folk music appropriate to the time period. With the exception of a few vintage tracks (such as Harry McClintock's 1928 single "Big Rock Candy Mountain"), most tracks are modern recordings.

1 James Carter & The Prisoners– Po Lazarus 4:30
2 Harry McClintock– Big Rock Candy Mountain 2:17
3 Norman Blake – You Are My Sunshine 4:24
4 Alison Krauss– Down To The River To Pray 2:53
5 The Soggy Bottom Boys– I Am A Man Of Constant Sorrow (Radio Station Version) 3:13
6 Chris Thomas King– Hard Time Killing Floor Blues 2:01
7 Norman Blake – I Am A Man Of Constant Sorrow (Instrumental) 4:30
8 The Whites– Keep On The Sunny Side 3:35
9 Alison Krauss And Gillian Welch– I'll Fly Away 3:57
10 Emmylou Harris, Alison Krauss And Gillian Welch– Didn't Leave Nobody But The Baby 1:58
11 Sarah, Hannah And Leah Peasall– In The Highways 1:36
12 The Cox Family– I Am Weary (Let Me Rest) 3:16
13 John Hartford– I Am A Man Of Constant Sorrow (Instrumental) 2:34
14 Ralph Stanley– O Death 3:21
15 The Soggy Bottom Boys– In The Jailhouse Now 3:36
16 The Soggy Bottom Boys– I Am A Man Of Constant Sorrow (With Band) 4:18
17 John Hartford– Indian War Whoop 1:30
18 Fairfield Four*– Lonesome Valley 4:08
19 The Stanley Brothers– Angel Band 2:18